Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To air the preparation of rootage films from human blood sample. ii.To learn the techniques of color the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that baksheesh in human blood sample under microscope. interjection: Leukocytes ar divided into granulocytes and agranulocytes. Granulocytes are also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we swirl on look really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are also pale, just about invisible and we will not see anything inside them. To be able to observe and recognize the o pposite kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa discoloration and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene blue (basic dye) and eosin ( sulphurous dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone affectionateness specimens.

The principle of Giemsa stain is the basic dye stain the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to enjoin types of leukocytes. By patch of the c ells we can observe the different types of ! unclouded blood cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microscopes 10)Oil Immersion 11)Pasteur pipettes 12)Sorensens...If you want to get a lavish essay, fix it on our website: OrderCustomPaper.com

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